How To Make a Tris Buffer

Here's How:

1. Calculate Moles of Tris Base

   Determine the number of moles of Tris base needed by multiplying the desired molar concentration of buffer by the volume of buffer being made.

   mol/L * L = moles needed

2. Calculate Mass of Tris Base

   Determine the mass of Tris base to weigh by multiplying the number of moles by the molecular weight (121.14 g/mol) of Tris.

   moles needed * g/mol = g

3. Dissolve Tris Base in Water

   Dissolve the required mass of Tris into a volume of deionized water approximately 1/3 of the desired volume of buffer to be made.

4. Adjust the pH

   Using a pH meter, titrate the solution of Tris with 1M hydrochloric acid (HCl) until the correct pH is reached.

5. Bring to Volume

   Add the TrisHCl mixture to a volumetric flask of the desired volume and add deionized water as required to complete the solution.

What You Need:

• Standard labware: beakers, flasks, graduated cylinders, stirbars etc.
• pH meter and probe.
• Stirring hotplate.
• Tris base powder and hydrochloric acid (HCl).