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Common pH Indicators

By Theresa Phillips, About.com

Colorimetric assay plate. ©2000 T. Phillips.

The following are some commonly used pH indicators that might be suitable for use in colorimetric bioassays for catalysis of redox reactions or other enzyme activities that cause pH changes in a solution.

Common Indicators for Biological Applications

Common nameChemical namepH range (pka)Color in AcidColor in Alkaline
Bromo-phenol bluetetrabromophenol-sulphone-phthalein3.0-4.6 (4.1)yellowblue
Methyl yellowDimethylamino-azo-benzene2.9-4.0 (3.3)redyellow
Methyl orangeDimethylamino-benzene sodium sulphonate3.1-4.4 (3.7)redorange
Congo redDiphenyl-bis-azo-1-naphthylamino-4-sulphonic acid3.0-5.0bluered
Bromo-cresol greenTetrabromo-m-cresol-sulphone-phthalein3.8-5.4 (4.7)yellowblue
Methyl redo-Carboxybenzene-azodimethyl-aniline4.2-6.3 (5.0)redyellow
Chlorophenol redDichloro-phenol-sulphone-phthalein4.8-6.4 (6.1)yellowred
4-Nitrophenol4-Nitrophenol5.6-7.6 (7.1)colorlessyellow
Bromocresol purpleDibromo-o-cresol-sulphone-phthalein5.2-6.8 (6.1)yellowpurple
Bromo-thymol blueDibromo-thymol-sulphone-phthalein6.0-7.6 (7.1)yellowblue
Phenol redPhenol-sulphone-phthalein6.8-8.4 (7.8)yellowred
Cresol red1-Cresolsulphone-phthalein7.2-8.8 (8.2)yellowred
Thymol blueThymol-sulphone-phthalein8.0-9.6 (8.9)yellowblue
Phenol-phthaleinPhenolphthalein8.3-10.0 (9.6)colorlessred

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