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Buffers and Media
A selection of recipes for buffers and media are provided. Instructions are given on how to prepare buffer solutions of any pH or molarity.
Make Sodium Citrate Buffer
How to Make Sodium Citrate Buffer
Make PBS Buffer
PBS is a commonly used buffer for immunohistochemical staining, used as a wash solution and for diluting antibodies.
Common pH Indicators
Some common pH indicators with near neutral pka values, and pH ranges making them suitable for use in colorimetric bioassays.
Dyes for Staining DNA
The features of different dyes on the market for DNA staining.
Make Lyophilization Buffer
In order to freeze dry a cell culture of yeast, bacteria or fungus, it is important to use the right media. This recipe is for lyophilization buffer, for use in preserving your cell lines for long term storage by freeze drying.
Make TBE Buffer
Simple instructions on how to make stock and working solutions of tris-borate-EDTA (TBE) buffer for gel electrophoresis separation of DNA.
Make TAE Buffer
Simple instructions on making stock and working solutions of Tris-acetate-EDTA (TAE) buffer for gel electrophoresis.
Make a Phosphate Buffer
Follow these instructions on how to make a phosphate buffer for biological applications at near neutral pH, or adapt them for making any other buffer using a weak acid / conjugate base pair.
Make a Tris Buffer
Learn how to calculate the amount of Tris to use and how much HCl to add to make a buffer of specific molarity and pH.