> Application> Buffers and Media

Common pH Indicators
Some common pH indicators with near neutral pka values, and pH ranges making them suitable for use in colorimetric bioassays.

Make Lyophilization Buffer
In order to freeze dry a cell culture of yeast, bacteria or fungus, it is important to use the right media. This recipe is for lyophilization buffer, for use in preserving your cell lines for long term storage by freeze drying.

Make TBE Buffer
Simple instructions on how to make stock and working solutions of tris-borate-EDTA (TBE) buffer for gel electrophoresis separation of DNA.

Make TAE Buffer
Simple instructions on making stock and working solutions of Tris-acetate-EDTA (TAE) buffer for gel electrophoresis.

Make a Phosphate Buffer
Follow these instructions on how to make a phosphate buffer for biological applications at near neutral pH, or adapt them for making any other buffer using a weak acid / conjugate base pair.

Make a Tris Buffer
Learn how to calculate the amount of Tris to use and how much HCl to add to make a buffer of specific molarity and pH.