Lab Techniques

By Theresa Phillips, About.com Guide to Biotech / Biomedical

Laboratory techniques and protocols for the biotechnology research lab.

1. Clone a Gene
2. Make a Buffer
3. Working With Enzymes

Clone a Gene

Molecular methods for gene cloning and theoretical explanations for how they work.

• Theory of PCR
• Why E. coli for Gene Cloning?
• Run a Gel to Separate DNA

Make a Buffer

Recipes and hints for making various the buffers used in the biotech lab.

• Phosphate Buffer
• Tris Buffer
• TAE Buffer for Gels
• TBE Buffer for Gels

Working With Enzymes

A description explaning what enzymes are and what different types of enzymes there are, in addition to explanations for the techniques and step-by-step protocols for working with enzymes in the biotechnology industry.

• What is an Enzyme?
• Restriction Enzymes
• Approaches to Enzyme Optimization